influence of glycosaminoglycans isolated from the rat liver with various stages of carbon tetrachloride-induced fibrosis upon collagen fibrillogenesis in vitro

نویسندگان

e.j kucharz from the department of internal medicine, silesian university school of medicine, tychy, poland.

چکیده

the influence of glycosaminoglycans (gag) on collagen fibrillogenesis in vitro was investigated. gag and collagen were isolated from the liver of rats treated with carbon tetrachloride at various time intervals (3-16 weeks). it was found that gag obtained from the fibrotic liver accelerated collagen fibril formation. this effect was more evident in a solution of type i collagen than type iii collagen. studies on the liver samples showed that collagen was relatively slowly liberated in a solution of acid from the fibrotic liver as compared to normal tissue. the observed phenomena were related to the stage of fibrosis, i.e., to the duration of carbon tetrachloride treatment. therefore, a role for gag alteration in the development of hepatic fibrosis is suggested.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

INFLUENCE OF GLYCOSAMINOGLYCANS ISOLATED FROM THE RAT LIVER WITH VARIOUS STAGES OF CARBON TETRACHLORIDE-INDUCED FIBROSIS UPON COLLAGEN FIBRILLOGENESIS IN VITRO

The influence of glycosaminoglycans (GAG) on collagen fibrillogenesis in vitro was investigated. GAG and collagen were isolated from the liver of rats treated with carbon tetrachloride at various time intervals (3-16 weeks). It was found that GAG obtained from the fibrotic liver accelerated collagen fibril formation. This effect was more evident in a solution of type I collagen than type II...

متن کامل

Therapeutic Potential of Mouse Bone Marrow Mesenchymal Stem Cells in Carbon Tetrachloride (Ccl4)-Induced Liver Fibrosis

Purpose: To study the effect of allogenic bone marrow mesenchymal stem cells (BMMSCs) transplantation on carbon tetrachloride-induced liver fibrosis in mice. Materials and Methods: Fifty five female NMRI mice were divided in 5 groups, and to induce liver fibrosis CCL4 intraperitonealy was injected 1ml/Kg twice a week for 8 weeks 106 allogenic BMMSCs were infused in cell therapy group via tail v...

متن کامل

Preventive effect of novel nanomissile of silymarin on liver injury induced by carbon tetrachloride in rat

Background and Aim: Silymarin, obtained from Silybum marianum has hepatoprotective effects and antioxidant activity and has been used in the treatment of liver diseases. Because of its poor aqueous solubility and low bioavailability its use has been limited. The aim of this study was to prepare a new and inexpensive formulation of silymarin using simple dissolution methods and compare its hepat...

متن کامل

Preventive effect of malotilate on carbon tetrachloride-induced liver damage and collagen accumulation in the rat.

Malotilate is a new drug suggested for use in chronic liver diseases. It is shown here to prevent liver damage caused by CCl4. The concomitant administration of malotilate with CCl4 significantly decreased hydroxyproline accumulation in the liver, liver prolyl 4-hydroxylase and liver and serum galactosylhydroxylysyl glucosyltransferase activities. However, it had no effect on the daily urinary ...

متن کامل

Bone morphogenetic protein-7 regulates Snail signaling in carbon tetrachloride-induced fibrosis in the rat liver

The aim of this study was to explore the molecular mechanism of the bone morphogenetic protein-7 (BMP-7) downregulation of Snail-mediated E-cadherin repression and mesenchymal-epithelial transition (MET) induction, since little is presently known about this issue. In this study, our aim was to elucidate the underlying mechanism by which cells acquire liver fibrosis characteristics after epithel...

متن کامل

Chronic restraint stress reduces carbon tetrachloride-induced liver fibrosis

Stress as a cofactor has been reported to affect the progression and severity of liver diseases. The present study investigated the effect of chronic restraint stress on carbon tetrachloride (CCl4)-induced liver fibrosis. A total of 30 male BALB/c mice were randomly divided into three groups: Oil-treated control group; CCl4-treated group; and CCl4 + restraint-treated group. CCl4 was administrat...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۹، شماره ۴، صفحات ۳۲۷-۳۳۲

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023